Cool tools: High-tech ways to visualize the invisible

From fluorescence imaging that lights up proteins to electron microscopy that pinpoints cellular structures, visualization techniques have literally and figuratively illuminated the inner workings of cells.

Still, the function of many proteins and molecules within cells as well as the details of certain critical cellular processes remain hard to see.

Thanks in part to research funded by the National Institutes of Health, the ability to peer further into the body at the cellular and subcellular levels is improving our understanding of cell structure, function and regulation — all of which are involved in both normal and disease states.

by Stephanie Dutchen, National Institute of General Medical Sciences

Fluorescence techniques

Fluorescence techniquesCell biologists use fluorescence imaging to study cellular structures and processes, often in living cells. Through the staining or tagging of DNA, proteins and other targets with a fluorescent dye, fluorescence imaging techniques have revealed mechanisms of cell division, embryonic development and nerve regeneration.

Biologists can now observe whole biological systems with a resolution of about 20 nanometers — 2,500 times smaller than the width of a single human hair. Researchers are also working to create new types of dyes, optically active materials, labeling strategies, fluorescent proteins and nanoparticles to produce brighter and more detailed images.

Fluorescence techniques image: One new approach to fluorescence imaging uses light to manipulate cell activity. In this video, a blue laser beam activates a protein called Rac1, which then stimulates the movement of a human cancer cell. The technique can turn Rac1 — and potentially many other proteins — on and off at exact times and places in living cells. Credit: Yi Wu, the Hahn lab, University of North Carolina.

Live cell imaging

Live cell imagingLive cell imaging allows researchers to watch individual cells or subcellular components while barely disrupting the cell. With the aid of high-resolution microscopes, cameras and fluorescent sensors, they can create time-lapse movies of cellular and molecular interactions.

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What they see can answer questions about gene expression, cell division (mitosis), structural changes during programmed cell death and more.

Live cell imaging image: This video of cells from an African clawed frog used live cell imaging to capture abnormal mitosis in action. Just one late-to-align chromosome delays anaphase, the stage when chromosomes segregate to the two ends of the cell. Credit: John Daum and Gary Gorbsky, Oklahoma Medical Research Foundation.

Single-molecule imaging

Single-molecule imagingBiologists use an array of tools to study single molecules, including molecular manipulation with optical tweezers, single-molecule fluorescence spectroscopy, and microscopy and scanning methods to map molecular surfaces. By observing a single molecule, researchers can avoid errors that result from averaging data across many molecules and explore details within complex molecular mixtures.

Single-molecule imaging graphic: One technique for single-molecule imaging, called FIONA (fluorescence imaging with one-nanometer accuracy), allowed researchers to find out which model for myosin V movement was correct. Myosin is a motor, or movement, protein that carries cargo across cells using filaments made of actin. They found that myosin “walks” along actin in a hand-over-hand movement.

Electron Microscopy

Electron microscopyElectron microscopy uses a beam of electrons to illuminate and magnify cells that have been stained and prepared on slides. Electron microscopes can achieve about 1,000 times greater magnification than light-based imaging and are useful for studying any basic cellular process as well as developing molecular tags for genetic studies or live cell imaging.

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They’ve been instrumental in finding the exact locations of complex molecular assemblies within cells and in determining the structures of proteins.

Electron microscopy image: Captured using a transmission electron microscope, this image of a thin section of a single cell shows distinct cellular compartments and the structures within them. Credit: Tina Carvalho, University of Hawaii.

Making sense of imaging data

Fluorescence speckle microscopyScientists use quantitative imaging techniques (which go by names like FRET, FRAP and speckle microscopy) to measure direct molecular interactions inside cells. The data teach them more about how proteins act together to produce cellular functions — something they can’t learn from analyzing individual molecules — as well as how proteins transition between activities.

The computers that sort through imaging data are programmed to recognize certain patterns and eliminate background noise, allowing efficient analysis of stacks of images.

Imaging data analysis image: Fluorescence speckle microscopy, which tags a fraction of a protein, can improve image focus and the visibility of structures and dynamics in thick regions of living cells. Here, speckle microscopy illuminates the intricate network of microtubule (yellow) and actin filament (purple) fibers that builds a cell’s structure. Credit: Torsten Wittmann, UCSF.

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